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KMID : 0380019950100010038
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Korean Journal of Biotechnology and Bioengineering
1995 Volume.10 No. 1 p.38 ~ p.44
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Expression of Heterologous Promoters in Aspergillus oryzae
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Hahm, Young Tae/함영태
Kim, Hee Chung/Batt, Carl A/김희정/Batt, Carl A
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Abstract
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The expression of Aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase (gpdA) and trpC promoters in A. oryzae were compared using E. coli lacZ gene fusions. The specific acitivities of the expressed E. coli Q galactosidase in A. oryzae transformants containing the A. nidulans gpdA promoter were around 2,000 units per ug of protein. The specific activities of transformants containing the A. nidulans trpC promoter were very low, ranging from 10.5 to 52.3 units per ug of protein. These results showed that the expression of the A. nidulans gpdA promoter in A. oryzae was approximately 70 times greater than the A. nidulans trpC promoter. In western blot analysis, immunoreactive bands of a similar molecular weight as the E. coli /9-galactosidase were observed in A. oryzae carrying the gpdA-lacZ fusion and to a lesser intensity in those carrying the trpC-lacZ fusion. Southern analysis showed that the higher expression of the gpdA-lacZ fusion as compared to the trpC-lacZ fusion was not due a greater number of integrated plasmids.
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